289-Fujimoto-MitralCell/Fig7_BarrelCortex_Grin1KO_RhoA_1
Author reviewedZ-series images of the barrel cortex of Grin1-KO and RhoA-overexpressed mouse at P14 in which layer 4 neurons were labeled
Project
Dataset ID
Fig7_BarrelCortex_Grin1KO_RhoA_1
Kind
Image data
Title
Z-series images of the barrel cortex of Grin1-KO and RhoA-overexpressed mouse at P14 in which layer 4 neurons were labeled
Description
Z-series images of the barrel cortex of Grin1-KO and RhoA-overexpressed mouse at P14 in which layer 4 neurons were labeled by tdTomato. Grin1 coding NR1, an essential subunit of NMDA receptor was knocked out by using CRISPR-Cas9 and in utero electroporation. In this experoment, CRISPR-resistant-pCAG-RhoA was also introduced by in utero electroporation. VGluT2 was visualized by immmunohistochemistry.
Folder Name
Fig7_BarrelCortex_Grin1KO_RhoA_1
File Format
.lif
File Size
40.7GB
File Separation Method
10 series of images
Figure Number
Fig7
Dimensions
2048x2048x97x3x1
X Scale
0.284 micrometer
Y Scale
0.284 micrometer
Z Scale
1.04 micrometer
T Scale
-
Channel
3 channel
Quantitative Data
-
Paper(s)
Biosample
Description
A fixed and cleared barrel cortex of P14 mouse in which Grin1 was knocked out. In addition, RhoA was overexpressed in the brain. tdTomato was expressed in layer 4 neurons.
Organism
Mus musculus (NCBITaxon:10090)
Strain
Cell
neuron (CL:0000540)
Cell line
-
MeSH
Intrinsic Variable
pCAX-hCas9 and the gRNA for Grin1 were introduced to knockout Grin1 by CRISPR-Cas9 method.
Extrinsic Variable
By using in utero electroporation, the authors electroporated pCAFNF-tdTomato, pCAFNF-RhoA, and pCAG-FLPo at E13.5.
Ontology
Anatomical Entity
-
UBERON
Biological Process
neuron development neuron remodeling (GO:0016322)
Cellular Component
cell body (GO:0044297)
dendrite (GO:0030425)
Molecular Function
-
MeSH Anatomy
MeSH BP
MeSH CC
MeSH MF
Genetics
Gene
-
Protein / Probe
-
Method / Genotype
transfection
electroporation
Gene
-
Protein / Probe
Vesicular glutamate transporter 2 / antibody
Method / Genotype
-
Gene
Grin1
Protein / Probe
-
Method / Genotype
gene knock out
electroporation
Gene
-
Protein / Probe
Transforming protein RhoA
Method / Genotype
transfection
electroporation
Treatments
Reagent / Compound
-
Concentration
-
Fold Dilution
-
Imaging Method
Imaging Method
confocal microscopy (FBbi:00000251)
Detection Method
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Visualization Method
tdTomatoFP
primary antibody plus labeled secondary antibody
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
formaldehyde fixed tissue
vibratome-sectioned tissue
tissue clearing
MeSH
Instrument
Body
Leica DMi8
Module
-
Light Source
-
Detector
-
Objective
Leica HC PL APO 20x/0,75 IMM CORR CS2
Filter Set
-
Dichroic
-