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289-Fujimoto-MitralCell/Fig7_BarrelCortex_Grin1KO_1 thumbnail

289-Fujimoto-MitralCell/Fig7_BarrelCortex_Grin1KO_1

Author reviewed
Z-series images of the barrel cortex of Grin1-KO mouse at P14 in which layer 4 neurons were labeled
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Project
289-Fujimoto-MitralCell
Dataset ID
Fig7_BarrelCortex_Grin1KO_1
Kind
Image data
Title
Z-series images of the barrel cortex of Grin1-KO mouse at P14 in which layer 4 neurons were labeled
Description
Z-series images of the barrel cortex of Grin1 knockout mouse at P14 in which layer 4 neurons were labeled by tdTomato. Grin1 coding NR1, an essential subunit of NMDA receptor was knocked out by using CRISPR-Cas9 and in utero electroporation. VGluT2 was visualized by immmunohistochemistry.
Folder Name
Fig7_BarrelCortex_Grin1KO_1
File Format
.lif
File Size
58.5GB
File Separation Method
13 series of images
Figure Number
Fig7
Dimensions
2048x2048x104x3x1
X Scale
0.284 micrometer
Y Scale
0.284 micrometer
Z Scale
1.04 micrometer
T Scale
-
Channel
3 channel
Quantitative Data
-
Paper(s)

Biosample

Description
A fixed and cleared barrel cortex of P14 mouse in which Grin1 was knocked out. tdTomato was expressed in layer 4 neurons. It was stained for VGluT2.
Organism
Mus musculus (NCBITaxon:10090)
Strain
ICR (https://www.jslc.co.jp/pdf/mouse/002_ICR2013.pdf)
Cell
neuron (CL:0000540)
Cell line
-
MeSH
Neurons (D009474)
Microscopy, Confocal (D018613)
Cerebral Cortex (D002540)
Neuronal Plasticity (D009473)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)
Intrinsic Variable
pCAX-hCas9 and the gRNA for Grin1 were introduced to knockout Grin1 by CRISPR-Cas9 method.
Extrinsic Variable
By using in utero electroporation, the authors electroporated pCAFNF-tdTomato and pCAG-FLPo at E13.5.

Ontology

Anatomical Entity
-
UBERON
barrel cortex (UBERON:0010415)
cerebral cortex (UBERON:0000956)
brain (UBERON:0000955)
Biological Process
neuron development neuron remodeling (GO:0016322)
Cellular Component
cell body (GO:0044297)
dendrite (GO:0030425)
Molecular Function
-
MeSH Anatomy
Neurons (D009474)
Microscopy, Confocal (D018613)
Cerebral Cortex (D002540)
Neuronal Plasticity (D009473)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)
MeSH BP
Neurons (D009474)
Microscopy, Confocal (D018613)
Cerebral Cortex (D002540)
Neuronal Plasticity (D009473)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)
MeSH CC
Neurons (D009474)
Microscopy, Confocal (D018613)
Cerebral Cortex (D002540)
Neuronal Plasticity (D009473)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)
MeSH MF
Neurons (D009474)
Microscopy, Confocal (D018613)
Cerebral Cortex (D002540)
Neuronal Plasticity (D009473)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)

Genetics

Gene
-
Protein / Probe
-
Method / Genotype
transfection electroporation
Gene
-
Protein / Probe
Vesicular glutamate transporter 2 / antibody
Method / Genotype
-
Gene
Grin1
Protein / Probe
-
Method / Genotype
gene knock out electroporation

Treatments

Reagent / Compound
-
Concentration
-
Fold Dilution
-

Imaging Method

Imaging Method
confocal microscopy (FBbi:00000251)
Detection Method
-
Visualization Method
tdTomatoFP primary antibody plus labeled secondary antibody
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
formaldehyde fixed tissue vibratome-sectioned tissue tissue clearing
MeSH
Neurons (D009474)
Microscopy, Confocal (D018613)
Cerebral Cortex (D002540)
Neuronal Plasticity (D009473)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)

Instrument

Body
Leica DMi8
Module
-
Light Source
-
Detector
-
Objective
Leica HC PL APO 20x/0,75 IMM CORR CS2
Filter Set
-
Dichroic
-