289-Fujimoto-MitralCell/Fig5G_Raichu-Rac1_NMDA20uM_2
Author reviewedFRET imaging of Rac1 activity in mitral cells after 20uM NMDA stimulation using the RaichuEV-Rac1 biosensor in the presence of TTX
Project
Dataset ID
Fig5G_Raichu-Rac1_NMDA20uM_2
Kind
Image data
Title
FRET imaging of Rac1 activity in mitral cells after 20uM NMDA stimulation using the RaichuEV-Rac1 biosensor in the presence of TTX
Description
Time-lapse images of Rac1 activity in olfactory bulb slice from P3 mouse. To stimurate NMDA receptors, the slice was treated with 20 micromolar NMDA and glycine during imaging. Rac1 activity was studied with RaichuEV-Rac1, a FRET biosensor for Rac1.
Folder Name
Fig5G_Raichu-Rac1_NMDA20uM_2
File Format
.oib
File Size
316MB
File Separation Method
-
Figure Number
Fig5G
Dimensions
844x428x1x2x229
X Scale
0.497 micrometer
Y Scale
0.497 micrometer
Z Scale
-
T Scale
5 seconds
Channel
2 channel
Quantitative Data
-
Paper(s)
Biosample
Description
Olfactory bulb slice expressing RaichuEV-Rac1. The acute slice excised from P3 mouse brain. The slice was treated with 20 micromolar NMDA and 40 micromolar glycine during imaging.
Organism
Mus musculus (NCBITaxon:10090)
Strain
Cell
mitral cell (CL:1001502)
Cell line
-
MeSH
Intrinsic Variable
-
Extrinsic Variable
pCAGGS-RaichuEV-Rac1 was introduced at E12 by in utero electroporation. The brain slice at P3 was treated with 20 micromolar NMDA and 40 micromolar glycine during imaging.
Ontology
Anatomical Entity
-
UBERON
olfactory bulb brain (UBERON:0000955)
Biological Process
postsynaptic signal transduction (GO:0098926)
Cellular Component
cell body (GO:0044297)
dendrite (GO:0030425)
Molecular Function
-
MeSH Anatomy
MeSH BP
MeSH CC
MeSH MF
Genetics
Gene
-
Protein / Probe
-
Method / Genotype
-
Treatments
Reagent / Compound
N-methyl-D-aspartic acid
Concentration
20 micromolar
Fold Dilution
-
Reagent / Compound
glycine
Concentration
40 micromolar
Fold Dilution
-
Reagent / Compound
tetrodotoxin
Concentration
1 micromolar
Fold Dilution
-
Imaging Method
Imaging Method
two-photon laser scanning microscopy (FBbi:00000254)
FRET (FBbi:00000367)
Detection Method
-
Visualization Method
RaichuEV-Rac1 composed of Ypet and ECFP
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
MeSH
Instrument
Body
Olympus-Evident FV1000MPE
Module
-
Light Source
-
Detector
-
Objective
Olympus, XLPLN25XXLPLN25XWMP, NA = 1.05, WD = 2.0
Filter Set
-
Dichroic
-