289-Fujimoto-MitralCell/Fig5G_DORA-RhoA_NMDA1uM_6
Author reviewedFRET imaging of RhoA activity in mitral cells after 1uM NMDA stimulation using the DORA-RhoA biosensor in the presence of TTX
Project
Dataset ID
Fig5G_DORA-RhoA_NMDA1uM_6
Kind
Image data
Title
FRET imaging of RhoA activity in mitral cells after 1uM NMDA stimulation using the DORA-RhoA biosensor in the presence of TTX
Description
Time-lapse images of RhoA activity in olfactory bulb slice from P3 mouse. To stimurate NMDA receptors, the slice was treated with 1 micromolar NMDA and glycine during imaging. RhoA activity was studied with DORA-RhoA, a FRET biosensor for RhoA.
Folder Name
Fig5G_DORA-RhoA_NMDA1uM_6
File Format
.oib
File Size
454MB
File Separation Method
-
Figure Number
Fig5G
Dimensions
1022x536x1x2x229
X Scale
0.497 micrometer
Y Scale
0.497 micrometer
Z Scale
-
T Scale
5 seconds
Channel
2 channel
Quantitative Data
-
Paper(s)
Biosample
Description
Olfactory bulb slice expressing DORA-RhoA. The acute slice excised from P3 mouse brain. The slice was treated with 1 micromolar NMDA and 40 micromolar glycine during imaging.
Organism
Mus musculus (NCBITaxon:10090)
Strain
Cell
mitral cell (CL:1001502)
Cell line
-
MeSH
Intrinsic Variable
-
Extrinsic Variable
pCAGGS-DORA-RhoA was introduced at E12 by in utero electroporation. The brain slice at P3 was treated with 1 micromolar NMDA and 40 micromolar glycine during imaging.
Ontology
Anatomical Entity
-
UBERON
olfactory bulb brain (UBERON:0000955)
Biological Process
postsynaptic signal transduction (GO:0098926)
Cellular Component
cell body (GO:0044297)
dendrite (GO:0030425)
Molecular Function
-
MeSH Anatomy
MeSH BP
MeSH CC
MeSH MF
Genetics
Gene
-
Protein / Probe
-
Method / Genotype
-
Treatments
Reagent / Compound
N-methyl-D-aspartic acid
Concentration
1 micromolar
Fold Dilution
-
Reagent / Compound
glycine
Concentration
40 micromolar
Fold Dilution
-
Reagent / Compound
tetrodotoxin
Concentration
1 micromolar
Fold Dilution
-
Imaging Method
Imaging Method
two-photon laser scanning microscopy (FBbi:00000254)
FRET (FBbi:00000367)
Detection Method
-
Visualization Method
DORA-RhoA composed of cpVenus and Cerulean
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
MeSH
Instrument
Body
Olympus-Evident FV1000MPE
Module
-
Light Source
-
Detector
-
Objective
Olympus, XLPLN25XXLPLN25XWMP, NA = 1.05, WD = 2.0
Filter Set
-
Dichroic
-