289-Fujimoto-MitralCell/Fig4J_Kir_caRhoA_3
Author reviewedZ-series images of Kir2.1- and caRhoA-overexpressed mouse brain at P6 in which mitral cells were labed by tdTomato
Project
Dataset ID
Fig4J_Kir_caRhoA_3
Kind
Image data
Title
Z-series images of Kir2.1- and caRhoA-overexpressed mouse brain at P6 in which mitral cells were labed by tdTomato
Description
Z-series images of Kir2.1-overexpressed mouse brain at P6 in which mitral cells were labed by tdTomato. A gene coding for the inward rectifying potassium channel, Kir2.1 was expressed to reduce the activity of mitral cells. In this experoment, constitutively active RhoA (caRhoA) was also introduced by in utero electroporation. The cleared olfactory bulb was stained with DAPI.
Folder Name
Fig4J_Kir_caRhoA_3
File Format
.lif
File Size
625MB
File Separation Method
-
Figure Number
Fig4J
Dimensions
2577x1025x124x2x1
X Scale
0.568 micrometer
Y Scale
0.569 micrometer
Z Scale
5 micrometer
T Scale
-
Channel
2 channel
Quantitative Data
-
Paper(s)
Biosample
Description
A fixed and cleared olfactory bulb of P6 mouse. tdTomato Kir2.1, and (constitutively active RhoA were expressed in mitral/tufted cells.
Organism
Mus musculus (NCBITaxon:10090)
Strain
Cell
mitral cell (CL:1001502)
Cell line
-
MeSH
Intrinsic Variable
-
Extrinsic Variable
By using in utero electroporation, the authors electroporated a Cre-dependent tdTomato plasmid (pCALNL-tdTomato), Cre plasmid (pCAG-Cre), and a plasmid for overexpression of caRhoA (constitutively active RhoA) and Kir2.1 (pCALNL-Kir2.1) at E12.
Ontology
Anatomical Entity
-
UBERON
olfactory bulb brain (UBERON:0000955)
Biological Process
neuron development (GO:0048666)
negative regulation of neuron remodeling (GO:1904800)
Cellular Component
cell body (GO:0044297)
dendrite (GO:0030425)
Molecular Function
-
MeSH Anatomy
MeSH BP
MeSH CC
MeSH MF
Genetics
Gene
-
Protein / Probe
Inward rectifier potassium channel 2
Method / Genotype
transfection
electroporation
Gene
-
Protein / Probe
-
Method / Genotype
transfection
electroporation
Gene
-
Protein / Probe
Transforming protein RhoA
Method / Genotype
transfection
electroporation
Treatments
Reagent / Compound
-
Concentration
-
Fold Dilution
-
Imaging Method
Imaging Method
confocal microscopy (FBbi:00000251)
Detection Method
-
Visualization Method
tdTomatoFP
4',6-diamidino-2-phenylindole (DAPI)
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
formaldehyde fixed tissue
vibratome-sectioned tissue
tissue clearing
MeSH
Instrument
Body
Leica DMi8
Module
-
Light Source
-
Detector
-
Objective
Leica HC PL APO 20x/0,75 IMM CORR CS2
Filter Set
-
Dichroic
-