289-Fujimoto-MitralCell/Fig4J_Kir_RhoA_1
Author reviewedZ-series images of Kir2.1- and RhoA-overexpressed mouse brain at P6 in which mitral cells were labed by tdTomato
Project
Dataset ID
Fig4J_Kir_RhoA_1
Kind
Image data
Title
Z-series images of Kir2.1- and RhoA-overexpressed mouse brain at P6 in which mitral cells were labed by tdTomato
Description
Z-series images of Kir2.1-overexpressed mouse brain at P6 in which mitral cells were labed by tdTomato. A gene coding for the inward rectifying potassium channel, Kir2.1 was expressed to reduce the activity of mitral cells. In this experiment, pCAG-RhoA was also introduced by in utero electroporation. The cleared olfactory bulb was stained with DAPI.
Folder Name
Fig4J_Kir_RhoA_1
File Format
.lif
File Size
661MB
File Separation Method
3 series
Figure Number
Fig4J
Dimensions
1024x1024x240x2x1
X Scale
0.568 micrometer
Y Scale
0.569 micrometer
Z Scale
5 micrometer
T Scale
-
Channel
2 channel
Quantitative Data
-
Paper(s)
Biosample
Description
A fixed and cleared olfactory bulb of P6 mouse. tdTomato Kir2.1, and RhoA were expressed in mitral/tufted cells.
Organism
Mus musculus (NCBITaxon:10090)
Strain
Cell
mitral cell (CL:1001502)
Cell line
-
MeSH
Intrinsic Variable
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Extrinsic Variable
By using in utero electroporation, the authors electroporated a pCAFNF-tdTomato and pCAG-FLPo, pCAFNF-RhoA and a plasmid for overexpression of Kir2.1 (pCALNL-Kir2.1) at E12.
Ontology
Anatomical Entity
-
UBERON
olfactory bulb brain (UBERON:0000955)
Biological Process
neuron development (GO:0048666)
negative regulation of neuron remodeling (GO:1904800)
Cellular Component
cell body (GO:0044297)
dendrite (GO:0030425)
Molecular Function
-
MeSH Anatomy
MeSH BP
MeSH CC
MeSH MF
Genetics
Gene
-
Protein / Probe
Inward rectifier potassium channel 2
Method / Genotype
transfection
electroporation
Gene
-
Protein / Probe
-
Method / Genotype
transfection
electroporation
Gene
-
Protein / Probe
Transforming protein RhoA
Method / Genotype
transfection
electroporation
Treatments
Reagent / Compound
-
Concentration
-
Fold Dilution
-
Imaging Method
Imaging Method
confocal microscopy (FBbi:00000251)
Detection Method
-
Visualization Method
tdTomatoFP
4',6-diamidino-2-phenylindole (DAPI)
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
formaldehyde fixed tissue
vibratome-sectioned tissue
tissue clearing
MeSH
Instrument
Body
Leica DMi8
Module
-
Light Source
-
Detector
-
Objective
Leica HC PL APO 20x/0,75 IMM CORR CS2
Filter Set
-
Dichroic
-