SSBD:database

289-Fujimoto-MitralCell/Fig4J_Kir_1 thumbnail

289-Fujimoto-MitralCell/Fig4J_Kir_1

Author reviewed
Z-series images of Kir2.1-overexpressed mouse brain at P6 in which mitral cells were labed by tdTomato
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Project
289-Fujimoto-MitralCell
Dataset ID
Fig4J_Kir_1
Kind
Image data
Title
Z-series images of Kir2.1-overexpressed mouse brain at P6 in which mitral cells were labed by tdTomato
Description
Z-series images of Kir2.1-overexpressed mouse brain at P6 in which mitral cells were labed by tdTomato. A gene coding for the inward rectifying potassium channel, Kir2.1 was expressed to reduce the activity of mitral cells. The cleared olfactory bulb was stained with DAPI.
Folder Name
Fig4J_Kir_1
File Format
.lif
File Size
661MB
File Separation Method
-
Figure Number
Fig4J
Dimensions
2715x1029x64x2x1
X Scale
0.568 micrometer
Y Scale
0.569 micrometer
Z Scale
5 micrometer
T Scale
-
Channel
2 channel
Quantitative Data
-
Paper(s)

Biosample

Description
A fixed and cleared olfactory bulb of P6 mouse. tdTomato and Kir2.1 were expressed in mitral/tufted cells.
Organism
Mus musculus (NCBITaxon:10090)
Strain
ICR (https://www.jslc.co.jp/pdf/mouse/002_ICR2013.pdf)
Cell
mitral cell (CL:1001502)
Cell line
-
MeSH
Neurons (D009474)
Microscopy, Confocal (D018613)
Olfactory Bulb (D009830)
Neuronal Plasticity (D009473)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)
Intrinsic Variable
-
Extrinsic Variable
By using in utero electroporation, the authors electroporated a Cre-dependent tdTomato plasmid (pCALNL-tdTomato), Cre plasmid (pCAG-Cre), and a plasmid for overexpression of Kir2.1 (pCALNL-Kir2.1) at E12.

Ontology

Anatomical Entity
-
UBERON
olfactory bulb brain (UBERON:0000955)
Biological Process
neuron development (GO:0048666)
negative regulation of neuron remodeling (GO:1904800)
Cellular Component
cell body (GO:0044297)
dendrite (GO:0030425)
Molecular Function
-
MeSH Anatomy
Neurons (D009474)
Microscopy, Confocal (D018613)
Olfactory Bulb (D009830)
Neuronal Plasticity (D009473)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)
MeSH BP
Neurons (D009474)
Microscopy, Confocal (D018613)
Olfactory Bulb (D009830)
Neuronal Plasticity (D009473)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)
MeSH CC
Neurons (D009474)
Microscopy, Confocal (D018613)
Olfactory Bulb (D009830)
Neuronal Plasticity (D009473)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)
MeSH MF
Neurons (D009474)
Microscopy, Confocal (D018613)
Olfactory Bulb (D009830)
Neuronal Plasticity (D009473)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)

Genetics

Gene
-
Protein / Probe
Inward rectifier potassium channel 2
Method / Genotype
transfection electroporation
Gene
-
Protein / Probe
-
Method / Genotype
transfection electroporation

Treatments

Reagent / Compound
-
Concentration
-
Fold Dilution
-

Imaging Method

Imaging Method
confocal microscopy (FBbi:00000251)
Detection Method
-
Visualization Method
tdTomatoFP 4',6-diamidino-2-phenylindole (DAPI)
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
formaldehyde fixed tissue vibratome-sectioned tissue tissue clearing
MeSH
Neurons (D009474)
Microscopy, Confocal (D018613)
Olfactory Bulb (D009830)
Neuronal Plasticity (D009473)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)

Instrument

Body
Leica DMi8
Module
-
Light Source
-
Detector
-
Objective
Leica HC PL APO 20x/0,75 IMM CORR CS2
Filter Set
-
Dichroic
-