289-Fujimoto-MitralCell/Fig4I_Grin1KO_RhoA_2
Author reviewedZ-series images of Grin1-knockout and RhoA-overexpressed mouse brain at P6 in which mitral cells were labed by tdTomato
Project
Dataset ID
Fig4I_Grin1KO_RhoA_2
Kind
Image data
Title
Z-series images of Grin1-knockout and RhoA-overexpressed mouse brain at P6 in which mitral cells were labed by tdTomato
Description
Z-series images of Grin1 knockout mouse brain at P6 in which mitral cells were labed by tdTomato. Grin1 coding NR1, an essential subunit of NMDA receptor was knocked out by using CRISPR-Cas9 and in utero electroporation. In this experoment, CRISPR-resistant-pCAG-RhoA was also introduced by in utero electroporation. The cleared olfactory bulb was stained with DAPI.
Folder Name
Fig4I_Grin1KO_RhoA_2
File Format
.lif
File Size
488MB
File Separation Method
3 series
Figure Number
Fig4I
Dimensions
1024x1024x244x2x1
X Scale
0.568 micrometer
Y Scale
0.569 micrometer
Z Scale
5 micrometer
T Scale
-
Channel
2 channel
Quantitative Data
-
Paper(s)
Biosample
Description
A fixed and cleared olfactory bulb of P6 mouse in which Grin1 was knocked out. In addition, RhoA was overexpressed in the brain. tdTomato was expressed in mitral/tufted cells.
Organism
Mus musculus (NCBITaxon:10090)
Strain
Cell
mitral cell (CL:1001502)
Cell line
-
MeSH
Intrinsic Variable
pCAX-hCas9 and the gRNA for Grin1 were introduced to knockout Grin1 by CRISPR-Cas9 method.
Extrinsic Variable
By using in utero electroporation, the authors electroporated a pCAFNF-tdTomato and pCAG-FLPo at E12. CRISPR-
resistant-pCAG-RhoA were also introduced.
Ontology
Anatomical Entity
-
UBERON
olfactory bulb brain (UBERON:0000955)
Biological Process
neuron development neuron remodeling (GO:0016322)
Cellular Component
cell body (GO:0044297)
dendrite (GO:0030425)
Molecular Function
-
MeSH Anatomy
MeSH BP
MeSH CC
MeSH MF
Genetics
Gene
Grin1
Protein / Probe
-
Method / Genotype
gene knock out
electroporation
Gene
-
Protein / Probe
-
Method / Genotype
transfection
electroporation
Gene
-
Protein / Probe
Transforming protein RhoA
Method / Genotype
transfection
electroporation
Treatments
Reagent / Compound
-
Concentration
-
Fold Dilution
-
Imaging Method
Imaging Method
confocal microscopy (FBbi:00000251)
Detection Method
-
Visualization Method
tdTomatoFP
4',6-diamidino-2-phenylindole (DAPI)
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
formaldehyde fixed tissue
vibratome-sectioned tissue
tissue clearing
MeSH
Instrument
Body
Leica DMi8
Module
-
Light Source
-
Detector
-
Objective
Leica HC PL APO 20x/0,75 IMM CORR CS2
Filter Set
-
Dichroic
-