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289-Fujimoto-MitralCell/Fig4I_Grin1KO_1

Author reviewed

Z-series images of Grin1 knockout mouse brain at P6 in which mitral cells were labed by tdTomato

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Project

289-Fujimoto-MitralCell

Dataset ID

Fig4I_Grin1KO_1

Kind

Image data

Title

Z-series images of Grin1 knockout mouse brain at P6 in which mitral cells were labed by tdTomato

Description

Z-series images of Grin1 knockout mouse brain at P6 in which mitral cells were labed by tdTomato. Grin1 coding NR1, an essential subunit of NMDA receptor was knocked out by using CRISPR-Cas9 and in utero electroporation.

Folder Name

Fig4I_Grin1KO_1

File Format

.lif

File Size

502MB

File Separation Method

3 series

Figure Number

Fig4I

Dimensions

1024x1024x251x2x1

X Scale

0.568 micrometer

Y Scale

0.569 micrometer

Z Scale

5 micrometer

T Scale

Channel

2 channel

Quantitative Data

Paper(s)

Biosample

Description

A fixed and cleared olfactory bulb of P6 mouse in which Grin1 was knocked out. tdTomato was expressed in mitral/tufted cells.

Organism

Mus musculus (NCBITaxon:10090)

Strain

ICR (https://www.jslc.co.jp/pdf/mouse/002_ICR2013.pdf)

Cell

mitral cell (CL:1001502)

Cell line

MeSH

Neurons (D009474)

Microscopy, Confocal (D018613)

Olfactory Bulb (D009830)

Neuronal Plasticity (D009473)

Cell Body (D066146)

Brain (D001921)

Dendrites (D003712)

Intrinsic Variable

pCAX-hCas9 and the gRNA for Grin1 were introduced to knockout Grin1 by CRISPR-Cas9 method.

Extrinsic Variable

By using in utero electroporation, the authors electroporated a pCAFNF-tdTomato and pCAG-FLPo at E12.

Ontology

Anatomical Entity

UBERON

olfactory bulb brain (UBERON:0000955)

Biological Process

neuron development (GO:0048666)

negative regulation of neuron remodeling (GO:1904800)

Cellular Component

cell body (GO:0044297)

dendrite (GO:0030425)

Molecular Function

MeSH Anatomy

Neurons (D009474)

Microscopy, Confocal (D018613)

Olfactory Bulb (D009830)

Neuronal Plasticity (D009473)

Cell Body (D066146)

Brain (D001921)

Dendrites (D003712)

MeSH BP

Neurons (D009474)

Microscopy, Confocal (D018613)

Olfactory Bulb (D009830)

Neuronal Plasticity (D009473)

Cell Body (D066146)

Brain (D001921)

Dendrites (D003712)

MeSH CC

Neurons (D009474)

Microscopy, Confocal (D018613)

Olfactory Bulb (D009830)

Neuronal Plasticity (D009473)

Cell Body (D066146)

Brain (D001921)

Dendrites (D003712)

MeSH MF

Neurons (D009474)

Microscopy, Confocal (D018613)

Olfactory Bulb (D009830)

Neuronal Plasticity (D009473)

Cell Body (D066146)

Brain (D001921)

Dendrites (D003712)

Genetics

Gene

Grin1

Protein / Probe

Method / Genotype

gene knock out electroporation

Gene

Protein / Probe

Method / Genotype

transfection electroporation

Treatments

Reagent / Compound

Concentration

Fold Dilution

Imaging Method

confocal microscopy (FBbi:00000251)

Detection Method

Visualization Method

tdTomatoFP 4',6-diamidino-2-phenylindole (DAPI)

Illumination Method

Source of Contrast

distribution of a specific protein

Imaged Parameter

fluorescence emission

Sample Preparation Method

formaldehyde fixed tissue vibratome-sectioned tissue tissue clearing

MeSH

Neurons (D009474)

Microscopy, Confocal (D018613)

Olfactory Bulb (D009830)

Neuronal Plasticity (D009473)

Cell Body (D066146)

Brain (D001921)

Dendrites (D003712)

Instrument

Body

Leica DMi8

Module

Light Source

Detector

Objective

Leica HC PL APO 20x/0,75 IMM CORR CS2

Filter Set

Dichroic