289-Fujimoto-MitralCell/Fig4G_P14_Grin1KO_3
Author reviewedZ-series images of Grin1 conditional knockout mouse brain at P14 in which mitral cells were labed by tdTomato
Project
Dataset ID
Fig4G_P14_Grin1KO_3
Kind
Image data
Title
Z-series images of Grin1 conditional knockout mouse brain at P14 in which mitral cells were labed by tdTomato
Description
Z-series images of Grin1 conditional knockout mouse brain at P14 in which mitral cells were labed by tdTomato. Grin1 codes NR1, an essential subunit of NMDA receptors.
Folder Name
Fig4G_P14_Grin1KO_3
File Format
.oib
File Size
606MB
File Separation Method
-
Figure Number
Fig4G
Dimensions
3070x1024x101x1x1
X Scale
0.497 micrometer
Y Scale
0.497 micrometer
Z Scale
10 micrometer
T Scale
-
Channel
1 channel
Quantitative Data
-
Paper(s)
Biosample
Description
A fixed and cleared olfactory bulb of P14 mouse in which Grin1 was knocked out sparsely. tdTomato was expressed in mitral/tufted cells.
Organism
Mus musculus (NCBITaxon:10090)
Strain
-
Cell
mitral cell (CL:1001502)
Cell line
-
MeSH
Intrinsic Variable
floxed-Grin1 mice, in which Grin1 was knocked out sparsely in mitral/tufted cells by expressing iCre.
Extrinsic Variable
By using in utero electroporation, the authors electroporated a pCAFNF-tdTomato, pCAG-FLPo, and pCAG-iCre at E12.
Ontology
Anatomical Entity
-
UBERON
olfactory bulb brain (UBERON:0000955)
Biological Process
neuron development neuron remodeling (GO:0016322)
Cellular Component
cell body (GO:0044297)
dendrite (GO:0030425)
Molecular Function
-
MeSH Anatomy
MeSH BP
MeSH CC
MeSH MF
Genetics
Gene
Grin1
Protein / Probe
-
Method / Genotype
transgenic / B6.129S4-Grin1^{tm2Stl}/J
Gene
-
Protein / Probe
-
Method / Genotype
transfection
electroporation / B6.129S4-Grin1^{tm2Stl}/J
Treatments
Reagent / Compound
-
Concentration
-
Fold Dilution
-
Imaging Method
Imaging Method
two-photon laser scanning microscopy (FBbi:00000254)
Detection Method
-
Visualization Method
tdTomatoFP
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
formaldehyde fixed tissue
vibratome-sectioned tissue
tissue clearing
MeSH
Instrument
Body
Olympus, FV1000MPE
Module
-
Light Source
-
Detector
-
Objective
Olympus, XLPLN25XSVMP, NA 1.0, WD 4.0 mm
Filter Set
-
Dichroic
-