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289-Fujimoto-MitralCell/Fig4B_MitTuf_cal_ctrl_9 thumbnail

289-Fujimoto-MitralCell/Fig4B_MitTuf_cal_ctrl_9

Author reviewed
Time-lapse calcium imaging of olfactory bulb of P6 mouse in vivo expressing GCaMP6f in mitral/tufted cells without expression of M/T-TeNT in the cells
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Project
289-Fujimoto-MitralCell
Dataset ID
Fig4B_MitTuf_cal_ctrl_9
Kind
Image data
Title
Time-lapse calcium imaging of olfactory bulb of P6 mouse in vivo expressing GCaMP6f in mitral/tufted cells without expression of M/T-TeNT in the cells
Description
Time-lapse calcium imaging of olfactory bulb of P6 mouse in vivo expressing GCaMP6f in mitral/tufted cells without expression of M/T-TeNT in the cells. The datasets of ctrl_8 to ctrl_10 were obtained from the same pup.
Folder Name
Fig4B_MitTuf_cal_ctrl_9
File Format
.oib
File Size
175MB
File Separation Method
-
Figure Number
Fig4B
Dimensions
256x256x1x1x1398
X Scale
1,99 micrometer
Y Scale
1.99 micrometer
Z Scale
-
T Scale
0.43 seconds
Channel
1 channel
Quantitative Data
-
Paper(s)

Biosample

Description
Live P6 mouse expressing GCaMP6f in mitral/tufted cells
Organism
Mus musculus (NCBITaxon:10090)
Strain
-
Cell
mitral cell (CL:1001502)
Cell line
-
MeSH
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Dendrites (D003712)
Time-Lapse Imaging (D059008)
Brain (D001921)
Intrinsic Variable
A mouse line Thy1-GCaMP6f (C57BL/6J-Tg (Thy1-GCaMP6f)GP5.11Dkim/J) in which GCaMP6f is expressed in neurons.
Extrinsic Variable
-

Ontology

Anatomical Entity
-
UBERON
olfactory bulb brain (UBERON:0000955)
Biological Process
calcium ion transport (GO:0006816)
Cellular Component
dendrite (GO:0030425)
Molecular Function
-
MeSH Anatomy
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Dendrites (D003712)
Time-Lapse Imaging (D059008)
Brain (D001921)
MeSH BP
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Dendrites (D003712)
Time-Lapse Imaging (D059008)
Brain (D001921)
MeSH CC
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Dendrites (D003712)
Time-Lapse Imaging (D059008)
Brain (D001921)
MeSH MF
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Dendrites (D003712)
Time-Lapse Imaging (D059008)
Brain (D001921)

Genetics

Gene
-
Protein / Probe
-
Method / Genotype
transgenic / C57BL/6J-Tg (Thy1-GCaMP6f)GP5.11Dkim/J

Treatments

Reagent / Compound
-
Concentration
-
Fold Dilution
-

Imaging Method

Imaging Method
two-photon laser scanning microscopy (FBbi:00000254)
time lapse microscopy (FBbi:00000249)
Detection Method
-
Visualization Method
GCaMP6f
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
MeSH
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Dendrites (D003712)
Time-Lapse Imaging (D059008)
Brain (D001921)

Instrument

Body
Olympus-Evident FV1000MPE
Module
-
Light Source
-
Detector
-
Objective
Olympus, XLPLN25XXLPLN25XWMP, NA = 1.05, WD = 2.0
Filter Set
-
Dichroic
-