SSBD:database

289-Fujimoto-MitralCell/Fig3E_MitTuf_cal_AP5CNQX_3 thumbnail

289-Fujimoto-MitralCell/Fig3E_MitTuf_cal_AP5CNQX_3

Author reviewed
Time-lapse calcium imaging of olfactory bulb slice with AP5 and CBQX treatment from P2-3 mouse expressing GCaMP3 in mitral/tufted cells
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Project
289-Fujimoto-MitralCell
Dataset ID
Fig3E_MitTuf_cal_AP5CNQX_3
Kind
Image data
Title
Time-lapse calcium imaging of olfactory bulb slice with AP5 and CBQX treatment from P2-3 mouse expressing GCaMP3 in mitral/tufted cells
Description
Time-lapse calcium imaging of olfactory bulb slice from P2-3 mouse expressing GCaMP3 in mitral/tufted cells. The brain slice was treated with AP5 and CNQX to inhibit the glutamate receptors.
Folder Name
Fig3E_MitTuf_cal_AP5CNQX_3
File Format
.oib
File Size
438MB
File Separation Method
-
Figure Number
Fig3E
Dimensions
256x256x1x1x3500
X Scale
1,99 micrometer
Y Scale
1.99 micrometer
Z Scale
-
T Scale
0.43 seconds
Channel
1 channel
Quantitative Data
-
Paper(s)

Biosample

Description
Olfactory bulb slices of M/T-GCaMP3 mouse. The acute slice excised from P2-3 mouse brain. The slice was treated with AP5 and CNQX.
Organism
Mus musculus (NCBITaxon:10090)
Strain
-
Cell
mitral cell (CL:1001502)
Cell line
-
MeSH
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Dendrites (D003712)
Time-Lapse Imaging (D059008)
Brain (D001921)
Intrinsic Variable
GCaMP3 was expressed in mitral/tufted cells
Extrinsic Variable
The bran slice was treated with 50 micromolar AP5 and 25 micromolar CNQX.

Ontology

Anatomical Entity
-
UBERON
olfactory bulb brain (UBERON:0000955)
Biological Process
calcium ion transport (GO:0006816)
Cellular Component
dendrite (GO:0030425)
Molecular Function
-
MeSH Anatomy
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Dendrites (D003712)
Time-Lapse Imaging (D059008)
Brain (D001921)
MeSH BP
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Dendrites (D003712)
Time-Lapse Imaging (D059008)
Brain (D001921)
MeSH CC
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Dendrites (D003712)
Time-Lapse Imaging (D059008)
Brain (D001921)
MeSH MF
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Dendrites (D003712)
Time-Lapse Imaging (D059008)
Brain (D001921)

Genetics

Gene
-
Protein / Probe
-
Method / Genotype
transgenic / C57BL/6Cr-Tg(Pcdh21-cre)Byoko B6;129S-Gt(ROSA)26Sortm38(CAG-GCaMP3)Hze/J

Treatments

Reagent / Compound
2-amino-5-phosphonopentanoic acid
Concentration
50 micromolar
Fold Dilution
-
Reagent / Compound
6-Cyano-7-nitroquinoxaline-2,3-dione
Concentration
25 micromolar
Fold Dilution
-

Imaging Method

Imaging Method
two-photon laser scanning microscopy (FBbi:00000254)
time lapse microscopy (FBbi:00000249)
Detection Method
-
Visualization Method
GCaMP3
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
MeSH
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Dendrites (D003712)
Time-Lapse Imaging (D059008)
Brain (D001921)

Instrument

Body
Olympus-Evident FV1000MPE
Module
-
Light Source
-
Detector
-
Objective
Olympus, XLPLN25XXLPLN25XWMP, NA = 1.05, WD = 2.0
Filter Set
-
Dichroic
-