289-Fujimoto-MitralCell/Fig3B_P2_MitTuf_cal
Author reviewedTime-lapse calcium imaging of olfactory bulb slice from P2 mouse expressing GCaMP3 in mitral/tufted cells
Project
Dataset ID
Fig3B_P2_MitTuf_cal
Kind
Image data
Title
Time-lapse calcium imaging of olfactory bulb slice from P2 mouse expressing GCaMP3 in mitral/tufted cells
Description
Time-lapse calcium imaging of olfactory bulb slice from P2 mouse expressing GCaMP3 in mitral/tufted cells.
Folder Name
Fig3B_P2_MitTuf_cal
File Format
.tif
File Size
44MB
File Separation Method
-
Figure Number
Fig3B
Dimensions
256x256x1x1x699
X Scale
1,99 micrometer
Y Scale
1.99 micrometer
Z Scale
-
T Scale
0.43 seconds
Channel
1 channel
Quantitative Data
-
Paper(s)
Biosample
Description
Olfactory bulb slices of M/T-GCaMP3 mouse. The acute slice excised from P2 mouse brain.
Organism
Mus musculus (NCBITaxon:10090)
Strain
-
Cell
mitral cell (CL:1001502)
Cell line
-
MeSH
Intrinsic Variable
GCaMP3 was expressed in mitral/tufted cells
Extrinsic Variable
-
Ontology
Anatomical Entity
-
UBERON
olfactory bulb brain (UBERON:0000955)
Biological Process
calcium ion transport (GO:0006816)
Cellular Component
dendrite (GO:0030425)
Molecular Function
-
MeSH Anatomy
MeSH BP
MeSH CC
MeSH MF
Genetics
Gene
-
Protein / Probe
-
Method / Genotype
transgenic / C57BL/6Cr-Tg(Pcdh21-cre)Byoko
B6;129S-Gt(ROSA)26Sortm38(CAG-GCaMP3)Hze/J
Treatments
Reagent / Compound
-
Concentration
-
Fold Dilution
-
Imaging Method
Imaging Method
two-photon laser scanning microscopy (FBbi:00000254)
time lapse microscopy (FBbi:00000249)
Detection Method
-
Visualization Method
GCaMP3
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
MeSH
Instrument
Body
Olympus-Evident FV1000MPE
Module
-
Light Source
-
Detector
-
Objective
Olympus, XLPLN25XXLPLN25XWMP, NA = 1.05, WD = 2.0
Filter Set
-
Dichroic
-