289-Fujimoto-MitralCell/Fig1O_P6_OSN-TeNT_3
Author reviewedZ-series images of P6 mouse olfactory bulb with expression of TeNT in OSNs, which received hand rearing
Project
Dataset ID
Fig1O_P6_OSN-TeNT_3
Kind
Image data
Title
Z-series images of P6 mouse olfactory bulb with expression of TeNT in OSNs, which received hand rearing
Description
Z-series images of P6 mouse brain in which a subset of mitral cells sparsely labed by tdTomato, and TeNT (tetanus toxin light chain) was expressed in OSNs (olfactory sensory neurons). The mouse was nourished until P6 by human hand, and the brain was sliced and cleared with SeeDB2. The slice was stained with DAPI.
Folder Name
Fig1O_P6_OSN-TeNT_3
File Format
.lif
File Size
466MB
File Separation Method
3series
Figure Number
Fig1O
Dimensions
1024x1024x233x2x1
X Scale
0.568 micrometer
Y Scale
0.568 micrometer
Z Scale
2 micrometer
T Scale
-
Channel
2 channel
Quantitative Data
-
Paper(s)
Biosample
Description
A fixed and cleared olfactory bulb of P6 mouse. tdTomato was expressed in mitral cells, and TeNT was expressed in OSNs
Organism
Mus musculus (NCBITaxon:10090)
Strain
-
Cell
mitral cell (CL:1001502)
Cell line
-
MeSH
Intrinsic Variable
A mouse line expressing tetanus toxin light chain (TeNT) in OSNs (OMP-Cre;R26-CAG-LoxP-TeNT, designated OSN-TeNT)
Extrinsic Variable
By using in utero electroporation, the authors electroporated a pCAFNF-tdTomato and pCAG-FLPo at E12.
Ontology
Anatomical Entity
-
UBERON
olfactory bulb brain (UBERON:0000955)
Biological Process
neuron development neuron remodeling (GO:0016322)
Cellular Component
cell body (GO:0044297)
dendrite (GO:0030425)
Molecular Function
-
MeSH Anatomy
MeSH BP
MeSH CC
MeSH MF
Genetics
Gene
-
Protein / Probe
-
Method / Genotype
transfection
electroporation / B6;129P2-Omp^{tm4(cre)Mom}/MomJ
B6;129S6-Gt(ROSA)26Sor<tm1.1(CAG-EGFP/TeNT)Imayo>
Gene
-
Protein / Probe
Tetanus toxin
Method / Genotype
transgenic / B6;129P2-Omp^{tm4(cre)Mom}/MomJ
B6;129S6-Gt(ROSA)26Sor<tm1.1(CAG-EGFP/TeNT)Imayo>
Gene
-
Protein / Probe
-4',6-diamidino-2-phenylindole (DAPI)
Method / Genotype
-B6;129P2-Omp^{tm4(cre)Mom}/MomJ
B6;129S6-Gt(ROSA)26Sor<tm1.1(CAG-EGFP/TeNT)Imayo>
Treatments
Reagent / Compound
-
Concentration
-
Fold Dilution
-
Imaging Method
Imaging Method
confocal microscopy (FBbi:00000251)
Detection Method
-
Visualization Method
tdTomatoFP
4',6-diamidino-2-phenylindole (DAPI)
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
formaldehyde fixed tissue
vibratome-sectioned tissue
tissue clearing
MeSH
Instrument
Body
Leica DMi8
Module
-
Light Source
-
Detector
-
Objective
Leica HC PL APO 20x/0,75 IMM CORR CS2
Filter Set
-
Dichroic
-