SSBD:database

289-Fujimoto-MitralCell/Fig1L_P14_OSN-TeNT_1

Author reviewed

Z-series images of P14 mouse olfactory bulb with expression of TeNT in OSNs

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Project

289-Fujimoto-MitralCell

Dataset ID

Fig1L_P14_OSN-TeNT_1

Kind

Image data

Title

Z-series images of P14 mouse olfactory bulb with expression of TeNT in OSNs

Description

Z-series images of P14 mouse brain in which a subset of mitral cells sparsely labed by tdTomato, and TeNT (tetanus toxin light chain) was expressed in OSNs (olfactory sensory neurons). The mouse was maternaly reared until P14, and the brain was sliced and cleared with SeeDB2.

Folder Name

Fig1L_P14_OSN-TeNT_1

File Format

.oif

File Size

785MB

File Separation Method

Figure Number

Fig1L

Dimensions

3070x1024x131x1x1

X Scale

0.497 micrometer

Y Scale

0.497 micrometer

Z Scale

10 micrometer

T Scale

Channel

1 channel

Quantitative Data

Paper(s)

Biosample

Description

A fixed and cleared olfactory bulb of P14 mouse. tdTomato was expressed in mitral cells, and TeNT was expressed in OSNs

Organism

Mus musculus (NCBITaxon:10090)

Strain

Cell

mitral cell (CL:1001502)

Cell line

MeSH

Neurons (D009474)

Microscopy, Fluorescence, Multiphoton (D036641)

Olfactory Bulb (D009830)

Neuronal Plasticity (D009473)

Cell Body (D066146)

Brain (D001921)

Dendrites (D003712)

Intrinsic Variable

A mouse line expressing tetanus toxin light chain (TeNT) in OSNs (OMP-Cre;R26-CAG-LoxP-TeNT, designated OSN-TeNT)

Extrinsic Variable

By using in utero electroporation, the authors electroporated a pCAFNF-tdTomato and pCAG-FLPo at E12.

Ontology

Anatomical Entity

UBERON

olfactory bulb brain (UBERON:0000955)

Biological Process

neuron development neuron remodeling (GO:0016322)

Cellular Component

cell body (GO:0044297)

dendrite (GO:0030425)

Molecular Function

MeSH Anatomy

Neurons (D009474)

Microscopy, Fluorescence, Multiphoton (D036641)

Olfactory Bulb (D009830)

Neuronal Plasticity (D009473)

Cell Body (D066146)

Brain (D001921)

Dendrites (D003712)

MeSH BP

Neurons (D009474)

Microscopy, Fluorescence, Multiphoton (D036641)

Olfactory Bulb (D009830)

Neuronal Plasticity (D009473)

Cell Body (D066146)

Brain (D001921)

Dendrites (D003712)

MeSH CC

Neurons (D009474)

Microscopy, Fluorescence, Multiphoton (D036641)

Olfactory Bulb (D009830)

Neuronal Plasticity (D009473)

Cell Body (D066146)

Brain (D001921)

Dendrites (D003712)

MeSH MF

Neurons (D009474)

Microscopy, Fluorescence, Multiphoton (D036641)

Olfactory Bulb (D009830)

Neuronal Plasticity (D009473)

Cell Body (D066146)

Brain (D001921)

Dendrites (D003712)

Genetics

Gene

Protein / Probe

Method / Genotype

transfection electroporation / B6;129P2-Omp^{tm4(cre)Mom}/MomJ B6;129S6-Gt(ROSA)26Sor<tm1.1(CAG-EGFP/TeNT)Imayo>

Gene

Protein / Probe

Tetanus toxin

Method / Genotype

transgenic / B6;129P2-Omp^{tm4(cre)Mom}/MomJ B6;129S6-Gt(ROSA)26Sor<tm1.1(CAG-EGFP/TeNT)Imayo>

Treatments

Reagent / Compound

Concentration

Fold Dilution

Imaging Method

two-photon laser scanning microscopy (FBbi:00000254)

Detection Method

Visualization Method

tdTomatoFP

Illumination Method

Source of Contrast

distribution of a specific protein

Imaged Parameter

fluorescence emission

Sample Preparation Method

formaldehyde fixed tissue vibratome-sectioned tissue tissue clearing

MeSH

Neurons (D009474)

Microscopy, Fluorescence, Multiphoton (D036641)

Olfactory Bulb (D009830)

Neuronal Plasticity (D009473)

Cell Body (D066146)

Brain (D001921)

Dendrites (D003712)

Instrument

Body

Olympus, FV1000MPE

Module

Light Source

Detector

Objective

Olympus, XLPLN25XSVMP, NA 1.0, WD 4.0 mm

Filter Set

Dichroic