SSBD:database

289-Fujimoto-MitralCell/Fig1HI_P28_Kir_3

Author reviewed

Z-series images of P28 mouse olfactory bulb with overexpression of Kir2.1

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Project

289-Fujimoto-MitralCell

Dataset ID

Fig1HI_P28_Kir_3

Kind

Image data

Title

Z-series images of P28 mouse olfactory bulb with overexpression of Kir2.1

Description

Z-series images of P28 mouse brain. By using in utero electroporation, the authors electroporated a Cre-dependent tdTomato plasmid (pCALNL-tdTomato) and a small amount of Cre plasmid (pCAG-iCre) at E12 to label a subset of mitral cells sparsely. pCALNL-Kir2.1 was also elactroporated to overexpress Kir2.1, and reduce the activity of mitral cells. .At P28 the brain was sliced and cleared with SeeDB2.

Folder Name

Fig1HI_P28_Kir_3

File Format

.oif

File Size

606MB

File Separation Method

Figure Number

Fig1HI

Dimensions

3070x1024x101x1x1

X Scale

0.497 micrometer

Y Scale

0.497 micrometer

Z Scale

10 micrometer

T Scale

Channel

1 channel

Quantitative Data

Paper(s)

Biosample

Description

A fixed and cleared olfactory bulb of P28 mouse. tdTomato and Kir2.1 were expressed in mitral cells.

Organism

Mus musculus (NCBITaxon:10090)

Strain

ICR (https://www.jslc.co.jp/pdf/mouse/002_ICR2013.pdf)

Cell

mitral cell (CL:1001502)

Cell line

MeSH

Neurons (D009474)

Microscopy, Fluorescence, Multiphoton (D036641)

Olfactory Bulb (D009830)

Neuronal Plasticity (D009473)

Cell Body (D066146)

Brain (D001921)

Dendrites (D003712)

Intrinsic Variable

Extrinsic Variable

By using in utero electroporation, the authors electroporated a Cre-dependent tdTomato plasmid (pCALNL-tdTomato), Cre plasmid (pCAG-Cre), and a plasmid for overexpression of Kir2.1 (pCALNL-Kir2.1) at E12.

Ontology

Anatomical Entity

UBERON

olfactory bulb brain (UBERON:0000955)

Biological Process

neuron development neuron remodeling (GO:0016322)

Cellular Component

cell body (GO:0044297)

dendrite (GO:0030425)

Molecular Function

MeSH Anatomy

Neurons (D009474)

Microscopy, Fluorescence, Multiphoton (D036641)

Olfactory Bulb (D009830)

Neuronal Plasticity (D009473)

Cell Body (D066146)

Brain (D001921)

Dendrites (D003712)

MeSH BP

Neurons (D009474)

Microscopy, Fluorescence, Multiphoton (D036641)

Olfactory Bulb (D009830)

Neuronal Plasticity (D009473)

Cell Body (D066146)

Brain (D001921)

Dendrites (D003712)

MeSH CC

Neurons (D009474)

Microscopy, Fluorescence, Multiphoton (D036641)

Olfactory Bulb (D009830)

Neuronal Plasticity (D009473)

Cell Body (D066146)

Brain (D001921)

Dendrites (D003712)

MeSH MF

Neurons (D009474)

Microscopy, Fluorescence, Multiphoton (D036641)

Olfactory Bulb (D009830)

Neuronal Plasticity (D009473)

Cell Body (D066146)

Brain (D001921)

Dendrites (D003712)

Genetics

Gene

Protein / Probe

Method / Genotype

transfection electroporation

Gene

Protein / Probe

Inward rectifier potassium channel 2

Method / Genotype

transfection electroporation

Treatments

Reagent / Compound

Concentration

Fold Dilution

Imaging Method

two-photon laser scanning microscopy (FBbi:00000254)

Detection Method

Visualization Method

tdTomatoFP

Illumination Method

Source of Contrast

distribution of a specific protein

Imaged Parameter

fluorescence emission

Sample Preparation Method

formaldehyde fixed tissue vibratome-sectioned tissue tissue clearing

MeSH

Neurons (D009474)

Microscopy, Fluorescence, Multiphoton (D036641)

Olfactory Bulb (D009830)

Neuronal Plasticity (D009473)

Cell Body (D066146)

Brain (D001921)

Dendrites (D003712)

Instrument

Body

Olympus, FV1000MPE

Module

Light Source

Detector

Objective

Olympus, XLPLN25XSVMP, NA 1.0, WD 4.0 mm

Filter Set

Dichroic