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289-Fujimoto-MitralCell/Fig1E_P6 thumbnail

289-Fujimoto-MitralCell/Fig1E_P6

Author reviewed
Z-series images of P6 mouse olfactory bulb, in which mitral cell and mature glutamatergic synapses were labeled
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Project
289-Fujimoto-MitralCell
Dataset ID
Fig1E_P6
Kind
Image data
Title
Z-series images of P6 mouse olfactory bulb, in which mitral cell and mature glutamatergic synapses were labeled
Description
Z-series images of P6 mouse brain. By using in utero electroporation at E12, the authors expressed tdTomato and PSD d1.2-GFP in mitral/tufted cells. At P3 the brain was sliced and cleared with SeeDB2,
Folder Name
Fig1E_P6
File Format
.lif
File Size
2GB
File Separation Method
-
Figure Number
Fig1E
Dimensions
2048x2048x318x2x1
X Scale
0.06 micrometer
Y Scale
0.06 micrometer
Z Scale
0.2 micrometer
T Scale
-
Channel
2 channel
Quantitative Data
-
Paper(s)

Biosample

Description
A fixed and cleared olfactory bulb of P6 mouse. tdTomato was expressed in mitral cells, and mature glumatergic postsynapses were labeled with PSD d1.2-GFP (a deletion mutant of PSD-95 fused to GFP).
Organism
Mus musculus (NCBITaxon:10090)
Strain
ICR (https://www.jslc.co.jp/pdf/mouse/002_ICR2013.pdf)
Cell
mitral cell (CL:1001502)
Cell line
-
MeSH
Neurons (D009474)
Microscopy, Confocal (D018613)
Olfactory Bulb (D009830)
Neuronal Plasticity (D009473)
Dendrites (D003712)
Brain (D001921)
Synapses (D013569)
Intrinsic Variable
-
Extrinsic Variable
By using in utero electroporation, the authors electroporated pCAFNF-tdTomato, pCAG-FLPo, and pCAFNF-d12PSD95-EGFP, a plasmid for PSD d1.2-GFP (a deletion mutant of PSD-95 fused to GFP) at E12.

Ontology

Anatomical Entity
-
UBERON
olfactory bulb brain (UBERON:0000955)
Biological Process
neuron development neuron remodeling (GO:0016322)
Cellular Component
dendrite (GO:0030425)
glutamatergic synapse (GO:0098978)
Molecular Function
-
MeSH Anatomy
Neurons (D009474)
Microscopy, Confocal (D018613)
Olfactory Bulb (D009830)
Neuronal Plasticity (D009473)
Dendrites (D003712)
Brain (D001921)
Synapses (D013569)
MeSH BP
Neurons (D009474)
Microscopy, Confocal (D018613)
Olfactory Bulb (D009830)
Neuronal Plasticity (D009473)
Dendrites (D003712)
Brain (D001921)
Synapses (D013569)
MeSH CC
Neurons (D009474)
Microscopy, Confocal (D018613)
Olfactory Bulb (D009830)
Neuronal Plasticity (D009473)
Dendrites (D003712)
Brain (D001921)
Synapses (D013569)
MeSH MF
Neurons (D009474)
Microscopy, Confocal (D018613)
Olfactory Bulb (D009830)
Neuronal Plasticity (D009473)
Dendrites (D003712)
Brain (D001921)
Synapses (D013569)

Genetics

Gene
-
Protein / Probe
Disks large homolog 4
Method / Genotype
transfection electroporation
Gene
-
Protein / Probe
-
Method / Genotype
transfection electroporation

Treatments

Reagent / Compound
-
Concentration
-
Fold Dilution
-

Imaging Method

Imaging Method
confocal microscopy (FBbi:00000251)
Detection Method
-
Visualization Method
tdTomatoFP GFP
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
formaldehyde fixed tissue vibratome-sectioned tissue tissue clearing
MeSH
Neurons (D009474)
Microscopy, Confocal (D018613)
Olfactory Bulb (D009830)
Neuronal Plasticity (D009473)
Dendrites (D003712)
Brain (D001921)
Synapses (D013569)

Instrument

Body
Leica DMi8
Module
-
Light Source
-
Detector
-
Objective
Leica HC PL APO 63x/1,30 GLYC CORR
Filter Set
-
Dichroic
-