Homologous centromere distributions in the first mitosis after whole-genome duplication in human HCT116 cells
Description
Fixed or live imaging data on spatial distributions of homologous centromeres labeled by specific FISH probes (for fixed imaging) or dCas9-EGFP with specific sgRNA (for live and fixed imaging). In dCas9-EGFP images, chromosomes and the centrosomes were also marked using H2B-mCherry and EGFP-GCP3, respectively. In som experiments, chromosomes and the centrosomes were fixed-stained with DAPI and anti-Cep192 antibody, respectively. Images show the distributions of labeled centromeres at different mitotic stages during the first mitosis after whole-genome duplication. dCas9-EGFP and EGFP-GCP3 signals are distinguished based on their location relative to mitotic chromosomes (dCas9-EGFP signals are on the chromosomes, while EGFP-GCP3 signals are out of the chromosome area). Anaphase/telophase images were used to analyze the frequency of nullisomic chromosome segregation (where certain segregated chromosome masses contain no homologous centromere signals), which differed depending on the types of whole-genome duplication occurring prior to the observed mitosis.
Masaya Inoko, Guang Yang, Yuki Tsukada, Ryota Uehara (2026) Sister chromatid separation determines the proliferative properties upon whole-genome duplication via homologous chromosome arrangement., Proceedings of the National Academy of Sciences of the United States of America, Volume 123, Number 16, pp. e2524135123
Persons
Ryota Uehara
Hokkaido University
Contact
Masaya Inoko
Hokkaido University
Imaging contributor, Quantitative data contributor