Intracellular signaling observed using giant Dictyostelium cells
Description
We developed a method to enlarge Dictyostelium discoideum cells by partial cytokinesis inhibition, generating multinucleated yet functional giant cells. These cells retained chemotactic signaling, polarity, and motility, enabling high-resolution live-cell imaging. Using fluorescent probes for cAMP and Ca2+, we uncovered a directional, front-to-rear propagation of cAMP signaling and a biphasic Ca2+ response coordinated with actin wave dynamics.
Submission date
2025-07-07
Release date
2026-01-21
Update date
-
License
CC BY license
Funding information
Japan Science and Technology Agency, JPMJSP2154, JPMJPR204B