SSBD:database

Name

ssbd-repos-000365

URL

DOI

Title

Slow diffusion and signal amplification on membranes regulated by phospholipase D

Description

Control of molecular diffusion is pivotal for highly fluidic membranes to serve as substrates for biochemical reactions and the self-assembly of molecular machinery driving membrane protrusions. Lateral diffusion in membranes depends on lipid composition, which is highly diverse and homeostatically controlled in living cells. Due to the complexity of the underlying processes, its impact on molecular diffusion remain largely unclear. In this study, we show that lipid diffusion in model membranes is markedly decreased in cytosolic extracts. The reduction in lipid diffusivity could be pharmacologically inhibited by targeting phospholipase D (PLD), and addition of PLD to membranes mimicked the reduction in diffusion. Phosphatidic acid, a direct product of PLD, diffused slowly in model membranes and reduced the diffusivity of surrounding lipids. Furthermore, we demonstrated that PLD specifically controls the lateral diffusion of a myristoylated protein in cells, possibly due to auxiliary electrostatic interactions between cationic residues located near the lipidated tail and anionic phospholipids. PLD controlled the size and lifetime of localized patches of phosphatidylinositol (3,4,5)-trisphosphates that specify regions of membrane protrusions. Overall, the results of this study suggest that PLD controls the lateral diffusion of certain membrane proteins, which play key roles in phosphoinositide signaling.

Submission date

2024-07-11

Release date

2026-05-13

Update date

-

License

CC BY-NC license

Funding information

This work was supported by grants from the Japan Science and Technology Agency (JST), CREST (JPMJCR1923, S. S.), FOREST (JPMJFR213Y, M. Y.), and the Japan Society for the Promotion of Science (JSPS) KAKENHI (JP22H01188, M. Y. and JP19H05801, S. S.).

File formats

tif

Data (repos)

18,102 files / 88.64 GiB

Bundle (zip)

12 files / 64.69 GiB