Raw data of an article “Repeated neural activations induce long-term structural plasticity of the nucleus” (Murano et al., unpublished)
Description
Activation of neurons triggers plastic changes in neural circuits that are essential for brain development, learning and memory, but overactivation can lead to pathological alterations. Here, we show that repeated neural activation induces long-term changes in the nuclear structure of neurons, persisting for two weeks. These alterations involve the disruption of nuclear lamina and epigenetic changes, which partially resemble the G2-M phase in cycling cells, accompanied by alterations in the transcriptome, chromatin accessibility, neural coding of information, and locomotor activity in mice. These cell cycle-like changes and hyper-locomotor activity were mitigated by in vivo gene knockout of cyclin B, a molecule essential for G2-M phase transition. Our results demonstrate that subchronic neural activation reinstates a cell cycle-like process, leading to chronic changes in neuronal function and abnormal behavior. Our findings provide novel insights into activity-dependent plastic changes in neuronal circuits, which may be relevant to the pathogenesis of neuropsychiatric disorders.
Submission date
2024-01-09
Release date
2026-07-14
Update date
-
License
CC BY license
Funding information
-
File formats
Raw and processed calcium fluorescence data in the original format of nVista system (inscopix; *.isxd files, paired with *.isxp metafile), extracted calcium signal traces of each neuron (*.csv), and trace of mouse movement in open field (*.tiff) are registered. The [*.isxd] data stored in a folder (*_data) are linked to a metafile (*.isxp) located in their parent folder, and these files can be opened with “Inscopix Data Processing Software‘’ available from inscopix (https://www.inscopix.com/). Processed datasets and codes used for the decoding analyses are available at GitHub (https://github.com/tmurano).