467-Yuswan-ClusteredApopERK
Author reviewedDynamics of epithelial tissue remodeling in Drosophila pupa
Project ID
467-Yuswan-ClusteredApopERK
Project SID
467
Title
Dynamics of epithelial tissue remodeling in Drosophila pupa
Description
Epithelial tissues undergo cell turnover both during development and for homeostatic maintenance. Removal of cells is coordinated with the increase in number of newly dividing cells to maintain barrier function of the tissue. In Drosophila metamorphosis, larval epidermal cells (LECs) are replaced by adult precursor cells called histoblasts. Removal of LECs must counterbalance the exponentially increasing adult histoblasts. Previous work showed that the LEC removal accelerates as endocytic activity decreases throughout all LECs. Here, we show that the acceleration is accompanied by a mode switching from isolated single-cell apoptosis to clustered ones induced by the endocytic activity reduction. The authors identify the epidermal growth factor receptor (EGFR) pathway via extracellular-signal regulated kinase (ERK) activity as the main components downstream of endocytic activity in LECs. The reduced ERK activity, caused by the decrease in endocytic activity, is responsible for the apoptotic mode switching. Initially, ERK is transiently activated in normal LECs surrounding a single apoptotic LEC in a ligand-dependent manner, preventing clustered cell death. Following the reduction of endocytic activity, LEC apoptosis events do not provoke these transient ERK up-regulations, resulting in the acceleration of the cell elimination rate by frequent clustered apoptosis. These findings contrasted with the common perspective that clustered apoptosis is disadvantageous. Instead, switching to clustered apoptosis is required to ccommodate the growth of neighboring tissues.
Submission Date
2025-09-10
Opened Date
-
Release Date
2026-10-01
Update Date
-
Kind
Image datasets
License
Project URL
-
Project DOI
-
Metadata Version
-
Template Version
3.03
Funding Information
This work was supported by the following
grants: Japan Science and Technology Agency - JST SPRING (JPMJSP2114) to K.Y., Core Research for Evolutional Science and Technology -JST CREST (JPMJCR1852), Japan Society for the Promotion of Science - MEXT KAKENHI (JP26114003, JP21H05255), JSPS KAKENHI (JP24687027, JP16H04800), Japan Agency for Medical Research and Development - AMED moonshot (22zf0127001h002) and the research grant for Astellas Foundation for Research on Metabolic Disorders to E. K., JSPS KAKENHI
(JP21K06144, JP21H05105, JP24H01405), JST
FOREST Program (J210000474), Takeda Science
Foundation, and Tohoku University FRIS Program for Creation of Interdisciplinary Research to D. U.
Total
36 files / 107.0 GB
Image datasets
zipped 18 files / 16.7 GB, raw image 18 files / 90.3 GB, total 36 files / 107.0 GB
Quantitative datasets
zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes
Biosample
Organism
Drosophila
(
NCBITaxon:7215
)
Strain
-
Cell
epidermal cell
(
CL:0000362
)
Cell line
-
MeSH
Ontology
Anatomical Entity
larval abdominal segment
(
FBbt:00001747
)
UBERON
pupa
(
UBERON:0003143
)
Biological Process
pupal development
(
GO:0035209
)
programmed cell death
(
GO:0012501
)
tissue morphogenesis
(
GO:0048729
)
Cellular Component
nucleus
(
GO:0005634
)
cytosol
(
GO:0005829
)
Molecular Function
-
Medical Subject Headings
Imaging Method
Method involved in biological imaging
confocal microscopy
(
FBbi:00000251
)
time lapse microscopy
(
FBbi:00000249
)
Paper(s)
People
Contact
Erina Kuranaga
Kyoto University
Graduate School and Faculty of Pharmaceutical Sciences
Laboratory for Histogenetic Dynamics
Daiki Umetsu
Osaka University
Department of Biological Sciences, Graduate School of Science
Laboratory of Cell Biology
Imaging dataset contributor
Kevin Yuswan (Kyoto University)
Xiaofei Sun (Kyoto University)
Quantitative dataset contributor
-
Image datasets
18
Quantitative datasets
0
Dataset List
Thumbnail
Dataset
Organism
Kind / Links
Time-lapse images of nls::CFP::Venus in control Drosophila pupa
Time-lapse images of nls::CFP::Venus in EGFR-RNAi expressing Drosophila pupa
Time-lapse images of nls::CFP::Venus in EGFR-lambda-top expressing Drosophila pupa
Time-lapse images of nls::CFP::Venus in control Drosophila pupa
Time-lapse images of nls::CFP::Venus in shi-TS Drosophila pupa
Time-lapse images of nls::CFP::Venus in shi-TS plus EGFR-lambda-top expressing Drosophila pupa
Time-lapse images of miniCic::mScarlet and nls::CFP::Venus in control Drosophila pupa
Time-lapse images of miniCic::mScarlet and nls::CFP::Venus in control Drosophila pupa
Time-lapse images of miniCic::mScarlet and nls::CFP::Venus in shi-TS Drosophila pupa
Time-lapse images of miniCic::mScarlet and nls::CFP::Venus in Rab5-Q88L Drosophila pupae.
Time-lapse images of miniCic::mScarlet and nls::CFP::Venus in rhgRNAi expressing Drosophila pupa
Time-lapse images of miniCic::mScarlet and nls::CFP::Venus in Drosophila pupa with EGFR knockdown
Time-lapse images of tubP-miniCic::
mScarlet and His3::mIFP for OptoDRONC-expressing Drosophila pupa
Time-lapse images of tubP-miniCic::
mScarlet and His3::mIFP without expressing OptoDRONC for control Drosophila pupa
Time-lapse images of miniCic::mScarlet and nls::CFP::Venus in Drosophila pupa with vein knockdown
Time-lapse images of miniCic::mScarlet and nls::CFP::Venus in Drosophila pupa with Keren knockdown
Time-lapse images of miniCic::mScarlet and nls::CFP::Venus in Drosophila pupa with gurken knockdown
Time-lapse images of miniCic::mScarlet and nls::CFP::Venus in Drosophila pupa with spitz knockdown