Detail of FigS7e_oocyte_siRrc1_Prc1


Project
SSBD:Repository
Title
Live imaging of oocytes expressing EGFP-Map4 (microtubules, green) and H2B-mCherry (chromosomes, magenta) after Prc1 RNAi with expression of siRNA-resistant Prc1.
Description
Live imaging of oocytes expressing EGFP-Map4 (microtubules, green) and H2B-mCherry (chromosomes, magenta) after Prc1 RNAi with expression of siRNA-resistant Prc1.
Release, Updated
2022-03-31
License
CC BY
Kind
Image data
File Formats
.lsm
Data size
3.0 GB

Organism
Mus musculus ( NCBI:txid10090 )
Strain(s)
-
Cell Line
-
Gene symbols
Prc1
Protein names
Map4, H2B
Protein tags
EGFP, mCherry

Datatype
-
Molecular Function (MF)
-
Biological Process (BP)
meiosis I nuclear membrane disassembly ( GO:0051079 )
Cellular Component (CC)
spindle ( GO:0005819 ) , kinetochore ( GO:0000776 ) , chromosome ( GO:0005694 )
Biological Imaging Method
confocal microscopy ( Fbbi:00000251 )
X scale
0.1037832 micrometer/pixel
Y scale
0.1037832 micrometer/pixel
Z scale
4 micrometer/slice
T scale
5 minutes per time interval

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
See details in Yoshida S, et. al. (2020) Nat Commun., 11(1):2652.
Related paper(s)

Contact
Tomoya S. Kitajima , RIKEN , Center for Biosystems Dynamics Research , Laboratory for Chromosome Segregation
Contributors

OMERO Dataset
OMERO Project
Source