Detail of Fig1C_141016_PIAS1_metaphaseII_12h


Project
SSBD:Repository
Title
Immunostaining images of a fusion protein with monomeric EGFP(mEGFP-PIAS1) exhibited a kinetochore-like localization pattern in mouse oocytes at metaphase I for 12 hours
Description
Immunostaining images of a fusion protein with monomeric EGFP(mEGFP-PIAS1) exhibited a kinetochore-like localization pattern in mouse oocytes at metaphase I for 12 hours
Release, Updated
2021-09-30
License
CC BY
Kind
Image data
File Formats
Data size
204.7 MB

Organism
Mus musculus ( NCBI:txid10090 )
Strain(s)
-
Cell Line
-
Protein names
PIAS1
Protein tags
mEGFP

Datatype
-
Molecular Function (MF)
-
Biological Process (BP)
centromeric sister chromatid cohesion ( GO:0070601 ) , meiotic cell cycle ( GO:0051321 )
Cellular Component (CC)
kinetochore ( GO:0000776 ) , chromosome ( GO:0005694 )
Biological Imaging Method
fluorescence microscopy ( Fbbi:00000246 )
X scale
0.0593047 micrometer/pixel
Y scale
0.0593047 micrometer/pixel
Z scale
1 micrometer/slice
T scale
-

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
See details in Ding Y, et. al. (2018) Current Biology, 28(10): 1661-1669.
Related paper(s)

Contact
Tomoya S. Kitajima , RIKEN , Center for Biosystems Dynamics Research , Laboratory for Chromosome Segregation
Contributors

OMERO Dataset
OMERO Project
Source