Detail of 100ms_40x_1.5x_ND8_timecourse_1_3_Pos4
Project
SSBD:Repository
Title
Fluorescence images of thioflavin-T with mCherry-Nter (N-terminal region of Argonaute2) in the presence of seeds of Nter aggregates.
Description
Fluorescence images of thioflavin-T, an amyloid detection reagent, with mCherry-Nter (N-terminal region of Argonaute2) in the presence of seeds of Nter aggregates. The seeds of Nter was prepared beforehand, and added to the experiment buffer. The addition of the seeds reduced the initial lag phase of the fluorescence intensity to less than 100 min after mixing monomeric Nter with ThT in aggregation buffer.
Release, Updated
2024-12-23
License
CC BY
Kind
Image data
File Formats
.tif
Data size
722.7 MB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
protein-containing complex assembly
(
GO:0065003
)
Cellular Component (CC)
Biological Imaging Method
X scale
0.108 micrometer/pixel
Y scale
0.108 micrometer/pixel
Z scale
-
T scale
10 minutes per time interval
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
See details in Narita H, et. al. BMC Biol. 2023 Apr 19;21(1):78.
Related paper(s)
Contact
Tomohiro Shima, Sotaro Uemura
, University of Tokyo, University of Tokyo
, Department of Biological Sciences, Graduate School of Science , Department of Biological Sciences, Graduate School of Science
Contributors
Haruka Narita
OMERO Project
Source