Detail of FigS5_closeCancer
Project
SSBD:Repository
Title
Time lapse images visualizing interactions between fibroblasts and breast cancer cells
Description
Time lapse images visualizing intercellular connections between tissue-resident cells (NIH-3T3 cells labelled with mCherry) and cancer cells (E0771 cells labelled with Azurite) by sGRAPHIC system. In sGRAPHIC system, GFP fluorescence is detected when sC-GR and N- GR interact with each other. E0771/sC-GR cells and NIH3T3/N-GR were co-cultured at 1:1. channel0; Azurite, channel1; GFP, channel2; mCherry
Release, Updated
2024-12-23
License
CC BY
Kind
Image data
File Formats
tif
Data size
439.1 MB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
cell-cell interaction
Cellular Component (CC)
cell-cell contact zone
Biological Imaging Method
X scale
227.94 micrometer/pixel
Y scale
227.94 micrometer/pixel
Z scale
-
T scale
10 minutes per time interval
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Minegishi M, Kuchimaru T, Nishikawa K, Isagawa T, Iwano S, Iida K, Hara H, Miura S, Sato M, Watanabe S, Shiomi A, Mabuchi Y, Hamana H, Kishi H, Sato T, Sawaki D, Sato S, Hanazono Y, Suzuki A, Kohro T, Kadonosono T, Shimogori T, Miyawaki A, Takeda N, Shintaku H, Kizaka-Kondoh S, Nishimura S Secretory GFP reconstitution labeling of neighboring cells interrogates cell-cell interactions in metastatic niches. Nat Commun. 2023 Dec 5;14(1):8031.
Related paper(s)
Contact
Takahiro Kuchimaru, Hirofumi Shintaku
, Jichi Medical University, RIKEN
, Graduate School of Medicine, Cluster for Pioneering Research
, Graduate School of Medicine, Cluster for Pioneering Research
Contributors
Misa Minegishi, Tomomi Shimogori, Atsushi Miyawaki, Hiroshi Hamana, Hiroyuki Kishi
OMERO Project
Source