Detail of FigS37_dualSRS_WingDisc


Project
SSBD:Repository
Title
Fluorescence image of membrane-located GFP and dual-color SRS (stimulated Raman scattering) imaging of bata-galactosidase activity in Drosophila wing disc
Description
The Drosophila wing disc (genotype: en-Gal4, UAS-mCD8-GFP/UAS-lacZ), in which beta-galactosidase (coded by lacZ) and membrane-localized GFP are expressed only in the posterior region, was incubated with 9C^{15}N-JCR-Bn-betaGal and betaGal-9^{13}C^{15}N-JCP.
Release, Updated
2024-11-25
License
CC BY
Kind
Image data
File Formats
.tif
Data size
1.4 MB

Organism
Drosophila ( NCBI:txid7215 )
Strain(s)
-
Cell Line
-

Datatype
-
Molecular Function (MF)
beta-galactosidase activity ( GO:0004565 )
Biological Process (BP)
Cellular Component (CC)
Biological Imaging Method
fluorescence microscopy ( Fbbi:00000246 )
stimulated Raman scattering (SRS) imaging
X scale
0.16 micrometer/pixel
Y scale
0.16 micrometer/pixel
Z scale
-
T scale
-

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
See details in Fujioka H, et. al. J Am Chem Soc. 2023 Apr 26;145(16):8871-8881.
Related paper(s)

Contact
Mako Kamiya , Tokyo Institute of Technology , Department of Life Science and Technology
Contributors

OMERO Dataset
OMERO Project
Source