Detail of FigS24_HEK293
Project
SSBD:Repository
Title
Stimulated Raman scattering (SRS) image of beta-Gal activity in live HEK293 (without LacZ expression) cells
Description
SRS (stimulated Raman scattering) imaging of beta-galactosidase activity in live cultured cells. HEK293 cells were incubated with 9C^{15}N-JCR-Bn-betaGal and 9CN-JR-Bn-betaGal in the presence of a beta-galactosidase-specific inhibitor (N-(n-nonyl)deoxygalactonojirimycin). Their peaks of the spectrum are 2190cm^{-1}, and 2227 cm^{-1} respectively.
Release, Updated
2024-11-25
License
CC BY
Kind
Image data
File Formats
.tif
Data size
977.1 KB
Datatype
-
Molecular Function (MF)
beta-galactosidase activity
(
GO:0004565
)
Biological Process (BP)
Cellular Component (CC)
Biological Imaging Method
stimulated Raman scattering (SRS) imaging
X scale
0.16 micrometer/pixel
Y scale
0.16 micrometer/pixel
Z scale
-
T scale
-
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
See details in Fujioka H, et. al. J Am Chem Soc. 2023 Apr 26;145(16):8871-8881.
Related paper(s)
Contact
Mako Kamiya
, Tokyo Institute of Technology
, Department of Life Science and Technology
Contributors
OMERO Project