Detail of Fig5d_FatBody_betaGal
Project
SSBD:Repository
Title
Fluorescence image of membrane and cytosolic GFP and stimulated Raman scattering (SRS) image of beta- galactosidase activity in Drosophila fat body
Description
A Drosophila fat body (genotype: hs-Flp122, UAS-mCD8-GFP; Ay-Gal4, UAS-GFP/UAS-lacZ), in which beta-galactosidase (coded by lacZ) GFP are co-expressed, was incubated with 9C^{15}N-JCR-Bn-betaGal in Schneider’s Drosophila medium containing 0.5% DMSO as a cosolvent for 3.5 h at room temperature. SRS signal was detected only in LacZ-positive cells.
Release, Updated
2024-11-25
License
CC BY
Kind
Image data
File Formats
.tif
Data size
978.5 KB
Datatype
-
Molecular Function (MF)
beta-galactosidase activity
(
GO:0004565
)
Biological Process (BP)
Cellular Component (CC)
Biological Imaging Method
X scale
0.16 micrometer/pixel
Y scale
0.16 micrometer/pixel
Z scale
-
T scale
-
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
See details in Fujioka H, et. al. J Am Chem Soc. 2023 Apr 26;145(16):8871-8881.
Related paper(s)
Contact
Mako Kamiya
, Tokyo Institute of Technology
, Department of Life Science and Technology
Contributors
OMERO Project
Source