Detail of Fig4c_A549
Project
SSBD:Repository
Title
SRS (stimulated Raman scattering) and fluorescence images of β-Gal, GGT, and DPP-4 activities in live A549 cells by using three kinds of Raman probes
Description
Three kinds of Raman probes were introduced into A549 cells; a dipeptidyl peptidase-4 (DPP-4)-targeted probe, 9^{13}C^{15}N-JCR- Bn-EP, a gamma-glutamyl transpeptidase (GGT)-targeted probe, 9^{13}CN-JCR-Bn-gGlu, and a beta-galactosidase-targeted probe, 9C^{15}N-JCR-Bn-betaGal. Their peaks of the spectrum are 2137 cm^{-1}, 2167 cm^{-1}, and 2190 cm^{-1} respectively. AVG_16PE.tif is probe fluorescence image
Release, Updated
2024-11-25
License
CC BY
Kind
Image data
File Formats
.tif
Data size
1.9 MB
Datatype
-
Molecular Function (MF)
beta-galactosidase activity
(
GO:0004565
)
Biological Process (BP)
Cellular Component (CC)
Biological Imaging Method
stimulated Raman scattering (SRS) imaging
X scale
0.16 micrometer/pixel
Y scale
0.16 micrometer/pixel
Z scale
-
T scale
-
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
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Filter set
-
Summary of Methods
See details in Fujioka H, et. al. J Am Chem Soc. 2023 Apr 26;145(16):8871-8881.
Related paper(s)
Contact
Mako Kamiya
, Tokyo Institute of Technology
, Department of Life Science and Technology
Contributors
OMERO Project
Source